How Molecular Weight Is Determined . Use Protein Molecular Weight when you wish to predict the location of a protein of interest on a gel in relation to a set of protein standards. DNA Molecular Weight Marker IV. We are pleased to offer OligoEvaluator , our online oligonucleotide sequence calculator that provides primer analysis values for PCR: Base count Molecular weight Extinction coefficient Oligo type µg/OD at 260 nm Length (base m MolBio; DNA Tools; Protein Tools; Calculators & Converters; Sequences; Proteins; Structures; Journals; Desk; Molecular Biology. DNA molarity calculator, oligo concentration. Oligo Calculator [使用説明] SEQUENCE BoxにOligo Sequenceを入力して、計算ボタンをクリックしてください。次のSequenceはリセットボタンをクリックしてください。 他のソフトから”コピー＆ペースト”で入力できます。 SEQUENCE Zoo?” will have everything but the “A” … Custom DNA Oligos; SYBR® Green Primers ; ... Molecular Weight Calculator. To calculate molecular weight, start by listing all elements present in the molecule. of dsDNA = (# nucleotides x 607.4) + 157.9, 1 A260 dsDNA = 50 µg/ml 1 A260 ssDNA = 37 µg/ml 1 A260 ssRNA = 40 µg/ml, DNA and RNA Molecular Weights and Conversions, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, Macromolecular Components of E. coli and HeLa Cells, Orders of Magnitude Prefixes for SI Units. In a copy of the Periodic Table of Elements, find the relative atomic mass of each element in the molecule. Molecular Weight Calculator This online calculator you can use for computing the average molecular weight (MW) of molecules by entering the chemical formulas (for example C3H4OH(COOH)3 ). Docking of small ligands into protein active sites of ssRNA (e.g., RNA Transcript): M.W. This calculator calculates the molecular weight for nucleotic acids (DNA or RNA), protein/peptide, or chemicals. DNA, RNA and oligo molecular weight (MW), mole, mass and molarity calculator 1) Select molecule type dsDNA ssDNA RNA olig A n, T n, C n, and G n are the number of each respective nucleotide within the polynucleotide. MWbase = molecular weight of the deprotonated nucleoside. For example, each chlorine atom contributes approximately 35.5 to the molecular weight. You can append copies of commonly used epitopes and fusion proteins using the supplied list. Protein Molecular Weight Protein Molecular Weight accepts one or more protein sequences or DNA coding sequences and calculates molecular weight. OligoSpec Calculator The OligoSpec™ calculator provides the physical properties for your oligo design. This can also be written as 650 g/mol (= molar mass) 1. Search DNA Molecular Weight (MW) Calculator. The radio boxes below the entry area drive the analysis. Enter the DNA sequence. Or you can choose by one of the next two option-lists, which contains a series of common organic compounds (including their chemical formula) and all the elements. Beware of garbage in/garbage out. If ssRNA is selected, all T’s are converted to U’s. Molecular Weight Calculations DNA Molecular Weight (for instance synthesized Oligonucleotides) Anhydrous Molecular Weight = (A n x 313.21) + (T n x 304.2) + (C n x 289.18) + (G n x 329.21) - 61.96 A n, T n, C n, and G n are the number of each respective nucleotide within the polynucleotide. The inverse of the molecular weight is the number of moles of template present in one gram of material. Protein Molecular Weight accepts a protein sequence and calculates the molecular weight. Also provides the atomic number, element name, base atomic weight and formula weight for the calcutated molecular formula. takes into account the M.W. Quickly calculate your oligo’s molecular weight and extinction coefficient. Company Telephone: Fax: Hours: Monday to Friday 8:30 - 17:30 PST (GMT-8) Location: 520 … Calculations are based on the isotopic composition of the elements. For DNA/RNA molecular weight, users have the option to input sequences or sequence length. Calculate the molecular weight of a formula. ªAddition of "159" to the M.W. Single-stranded sequences are assumed to have a 5' phosphate. Oligo Calculator tool calculates melting temperature (Tm), molecular weight (MW), Millimolar Extinction Coeff (OD/μmol, µg/OD) for oligos For RNA type: Standard RNA Bases (A, C, … Calculate molecular weight and GC content from a DNA or RNA sequence: This program will take a nucleic acid sequence and calculate the molecular weight and GC content. If you have a large DNA molecule, you will probably cut it into smaller fragments using a so-called DNA restriction enzyme. The average weight of a single DNA base pair (bp) is 650 daltons. Experimental details will help us provide an accurate quote and timeline estimate. This means that one mole of a bp weighs 650 g and that the molecular weight of any double stranded DNA template can be estimated by taking the product of its length (in bp) and 650. Calculation of chemical formula of DNA oligos, and molecular weight; difference between molecular weight and molecular mass; mass spectrometry of oligonucleotides Call us: +44 (0)23 80596778 / Request a quote online Molecular weight is measured in Atomic Mass Units, usually expressed as u or amu. GenScript provides a comprehensive range of online bioinformatics tools for Molecular Biology, Peptide, and Protein Researchers. Here are the steps you might follow. Fasta format is supported, but if more than one sequence is entered only the first sequence is analyzed. 1. Also provides the atomic number, element name, base atomic weight and formula weight for the calcutated molecular formula. In order to calculate the In this instance, the oligonucleotide is often referred to as a primer, or a short piece of DNA that binds to its complementary target … Molecular Mass Calculator. Choose a DNA, RNA, genome editing, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. Protein Molecular Weight You can append copies of commonly used epitopes and fusion proteins using the supplied list. Calculate DNA molecular weight. Please right click in "sequence tab" to insert internal modification. No phosphate is present at the 5' end of strands made by primer extension. Formula: This web application calculates the molecular mass (average, monoisotopic, and nominal), the elemental composition, and the mass distribution spectrum of a molecule given by its chemical formula, relative element weights, or sequence. This means that one mole of a bp weighs 650 g and that the molecular weight of any double stranded DNA template can be estimated by taking the product of its length (in bp) and 650. Method Description, Microbial Identification Service: Applications, Linear DNA: µg to pmol of Ends Calculator, Pharm/Tox Testing, IC50 for 100+ Cancer Cell Lines, 80 Xenograft Models: Anti-Tumor Activity Test In Vivo, Liposome Encapsulation Services: siRNA, mRNA, DNA, Stable RNAi Cell Line Development: Gene Knockdown, In Vivo Toxicology Service (Mouse, Rat), LD50 Study, In Vivo siRNA Delivery / Tissue Targeting (In Vivo RNAi), siRNA Transfection / RNAi Cell-based Library Screening, TET-inducible RNAi Gene Knockdown Cell Lines, Gene Expression Analysis (RT-PCR) Services, Cell Banking Service: Master Cell Bank Generation, Complete List of Altogen Xenograft Animal Models. DNA Molecular Weight Marker III, DIG-labeled. Isotope Molecular Weight and Labeled Liquid Density Calculator . allow undercut. One dalton is 1.67 x 10-24grams, so the human genome weighs 3.59 x 10-12 grams (10-12 grams is also If the DNA is single-stranded, then multiply the number of bases by the average mass of a single base (330 g/mol) to get the approximate mass of the whole molecule. Note: The nucleic acids (DNA, RNA and oligo) solutions with different concentration has different ability aborbing light.When the light wave is 260 nm, the Absorbance of light and the nucleic acid concentration is calculated as: C For all DNA You can append copies of commonly used epitopes and fusion proteins using the supplied list. 2. Options average mass ... Options average mass monoisotopic mass negative mode positive mode. DNA RNA. 1) ... Oligonucleotides composed of DNA (deoxyoligonucleotides) are often used in the polymerase chain reaction (PCR), a procedure that can be employed to amplify almost any piece of DNA. Free DNA and RNA Molecular Weight Calculator from EnCor Biotechnology Inc. The program will ignore numbers, spaces or characters like B or Z which do not correspond to one of the 4 DNA bases (G, T, A and C) and the additional RNA base (U). Approximate M.W. Protein Molecular Weight accepts one or more protein sequences and calculates molecular weight. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs. How to use this tool 1. DNA Molecular Weight Marker IX. DNA GC content calculator. Nucleic Acid Molecular Weight Conversions Exact M.W. dna to mrna converter. Tools: Protein Molecular Weight Calculator: Protein Molecular Weight accepts a protein sequence and calculates the molecular weight. DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. DNA/RNA/Protein/Chemical Molecular Weight Calculator. The molecular weight, max and min estimates will be equal for sequence that contains no ambiguous bases. Explanation: Step 1. Locked … DNA restriction If you have a large DNA molecule, you will probably cut it into smaller fragments using a so-called DNA restriction enzyme. Mass deficiency due to chemical … of ssDNA = (# nucleotides x 303.7) + 79.0 M.W. The OligoCalc DNA MW calculations assume that there is not a 5' monophosphate) Anhydrous Molecular Weight = (A n x 313.21) + (T n x 304.2) + (C n x 289.18) + (G n x 329.21) - 61.96 A n, T n, C n, and G n are the number of each respective nucleotide within the polynucleotide. This calculation is based on the assumption that the average weight of a base pair (bp) is 650 Daltons. Calculate molecular weight and GC content from a DNA or RNA sequence: This program will take a nucleic acid sequence and calculate the molecular weight and GC content. Calculate the molar mass of the fragment. of ssRNA = (# nucleotides x 320.5) + 159.0 M.W. Mass spectrometry is commonly used to find the molecular mass of small to medium-sized molecules. If only DNA length is given, the molecular weight is calculated as: MW = DNA Length (bp) × DNA/RNA base weight The approx. Molecular Weight Calculator. Oligo Calculator tool calculates melting temperature (Tm), molecular weight (MW), Millimolar Extinction Coeff (OD/μmol, µg/OD) for oligos ... (Tm), molecular weight (MW), Millimolar Extinction Coeff (OD/μmol, μg/OD) for oligos. The molecular weight or molar mass of any double stranded DNA fragment can therefore be calculated by multiplying its length (in bp) by 650 and the answer will be expressed as daltons or g/mol Molecular Weight Molecular Weight Scan Mass Spectrometry Result: Target Mass All Trypsin Chymotrypsin V8 Protease Percent Difference Maximum Difference (Daltons) Min Max : Mass Range (Daltons) Calculate Chemistry Matter Atomic Mass. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text The IDT free, online OligoAnalzyer tool, accessed from the SciTools tab on the IDT website (www.idtdna.com/scitools), will analyze an input sequence and provide its molecular weight, as well as GC content, melting temperature, µg/OD, nmole/OD, and extinction coefficient. = (An x 313.2) + (Tn x 304.2) + (Cn x 289.2) + (Gn x 329.2) + 79.0ª An, Tn, Cn, and Gn are the number of each respective nucleotide within the polynucleotide. Empirical data on the molecular weight of a compound depends on the size of the molecule in question. Gel electrophoresis Then you will place properly prepared samples of the nucleic acid solution in the wells of an agarose gel electrophoresis system and apply a voltage for a specified amount of time. dna to rna convertor, dna to rna sequence converter. Calculating the molecular weight The molecular weight can be calculated from the chemical formula by using standard values of the molecular weight of each atom. Formula to calculate molecular weight, concentrations, spectral conversion and various other physical properties of nucleotides, DNA, and plasmids. This calculator will estimate the molecular weight of any string entered into the text box after removing all characters that do not correspond to the list of standard bases. Please Paste the DNA Sequences: Following are the results: Please send the details of your project to info@altogenlabs.com, or call 512-433-6177 and we will be happy to provide an immediate price quote. Genetic Code; ... Oligonucleotide Concentration C (µM or pmol/µl) = A260 / (0.01 x N) C (ng/ml) = (A260 x MW) / (0.01 x … Compute pI/Mw is a tool which allows the computation of the theoretical pI (isoelectric point) and Mw (molecular weight) for a list of UniProt Knowledgebase (Swiss-Prot or TrEMBL) entries or for user entered sequences []. MWMod = molecular weight of the deprotonated form of each individual modification nPO2 = total count of internal phosphates, i.e., nbases + … For example, use the formula CH4 for methane, which comprises 1 atom of carbon and 4 atoms of hydrogen. This calculator determines the molecular weight of an amino acid sequence. First, the vector itself does not play a role in the molecular weight of your gene of interest, a vector is only vector. calculated is valid at physiological pH. Quick Links. of ssRNA (e.g., RNA Transcript): M.W. Molecular weight of a double-stranded DNA molecule = (# of basepairs x 650 daltons) Total weight of the human genome = 3.3 x 109 bp x 650Da = 2.15 X 1012 Da. This calculator calculates the molecular weight for nucleotic acids (DNA or RNA), protein/peptide, or chemicals. How do you calculate molecular weight of DNA? ªAddition of "79.0" to the M.W. This online calculator you can use for computing the average molecular weight (MW) of molecules by entering the chemical formulas (for example C3H4OH(COOH)3 ). Input the molecular formula and the weight is calculated. = (An x 329.2) + (Un x 306.2) + (Cn x 305.2) + (Gn x 345.2) + 159ª For DNA/RNA molecular weight, users have the option to input sequences or sequence length. DNA/RNA molarity Calculator: M = w / MW Where: M: DNA molarity, in mol w: DNA weight, in g MW: DNA molecular weight, in g/mol If only DNA length is given, the molecular weight is calculated as: MW = DNA … Average weight of a DNA basepair (sodium salt) = 650 daltons (1 dalton equals the mass of a single hydrogen atom, or1.67 x 10-24grams) Molecular weight of a double-stranded DNA molecule = (# of basepairs x 650 daltons) Total weight of the human genome = 3.3 x 109 bp x 650Da = 2.15 X 1012 Da. ... N is the number of nucleotides and 660 p g p … The molecular mass, often called the molecular weight (MW), is the weight of all atoms in a given molecular formula. Protein Molecular Weight accepts one or more protein sequences or DNA coding sequences and calculates molecular weight. = (An x 329.2) + (Un x 306.2) + (Cn x 305.2) + (Gn x 345.2) + 159ª An, Un, Cn, and Gn are the number of each respective nucleotide within the polynucleotide. calculating plasmid concentration from a260. number of bases in dna. Step 1. Please send the details of your project to info@altogenlabs.com, or call 512-433-6177 and we will be happy to provide an immediate price quote. Or you can choose by one of the next two option-lists, which contains a series of common organic compounds (including their chemical formula) and all the elements. When submitting a protein (amino acid) sequence, you can append copies of commonly used epitopes and fusion proteins using the supplied list. 's of Nucleic Acids: M.W. 1 Product Result | Match Criteria: Product Name, Description 11418009001 ; Roche pricing. Then, count the atoms using the molecular formula. 1 Product Result | Match Criteria: Product Name, Property, Description 11218603910 ; Roche pricing. The weight of larger molecules and macromolecules (e.g., DNA, proteins) is found using light scattering and viscosity. Calculate the molecular weight of a formula. DNA base pair molarity calculator. of a 5' triphosphate. This is the same as saying that one mole of a bp weighs 650 g 1. Chemical/biochemical pocket companion designed by the editors of Current Protocols. Thermo Fisher Scientific, See our nucleic acid isolation and analysis products, Exact M.W. Calculate DNA MW. Use Protein Molecular Weight when you wish to predict the location of a protein of interest on a gel in relation to a set of protein standards. of ssDNA (e.g., Oligonucleotides): M.W. You can append copies of commonly used epitopes and fusion proteins using the supplied list. Molecular weight of a DNA oligonucleotide (sodium salt, pH ≥ 7): MW = (N A x 335.2) + (N C x 311.2) + (N G x 351.2)+ (N T x 326.2) + P where: N X = number of residues of base x in the oligonucleotide Molecular weight For N- and C DNA, RNA and oligo molecular weight (MW), mole, mass and molarity calculator. We use cookies to optimize site functionality and give you the best possible experience. Press calculate to display the molecular weight. DNA concentration calculator. Calculation. 1 Answer Ernest Z. Aug 13, 2016 Here are the steps you might follow. takes into account the 5' monophosphate left by most restriction enzymes. Use Protein Molecular Weight when you The molecular weight of water is 18.02 g/mol, while it could be millions for many complex organic molecules. Mongo Oligo Mass Calculator v2.08. Zap! For all DNA options, U’s are converted to T’s. Number of DNA molecules calculation. DNA Molecular Weight (for instance synthesized Oligonucleotides) Anhydrous Molecular Weight = (A n x 313.21) + (T n x 304.2) + (C n x 289.18) + (G n x 329.21) - 61.96 . DNA restriction. Degenerate codes can be accepted. by Christoph Gohlke. User residue 'N' base anion mass sugar add mass Output close button output summary sequence by 3 10 50 extended output show … Molecular Weight Calculations. To use the calculator, you will need the Adobe Flash Player plug-in installed and activated in your browser. Exact M.W. double strand DNA base weight is 650 Dalton, for single strand is 330, for RNA is 340 Dalton. DNA Molecular Weight (typically for synthesized DNA oligonucleotides. M.W. Submit ... Help; Add rows For DNA type: Standard DNA Bases (A, C, G, T) Mixed Bases, expressed in UPPERCASE. How do I calculate the molecular weight of an oligo? The string “Zip. Often, DNA molecular weight markers and ladders come in two formats: pre-mixed with loading dye, or with the loading dye separate. The program will ignore numbers, spaces or characters like B or Z which Sequence (5'-->3') Memories. Weight Calculator and enjoy it on your iPhone, iPad, and iPod touch. Input the molecular formula and the weight is calculated. Download DNA/RNA/Protein and General Mol. Experimental details will help us provide an accurate quote and timeline estimate. Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. The subtraction of 61.96 gm/mole from the oligonucleotide molecular weight takes into account the … DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. DNA Molecular Weight: DNA Molecular Weight accepts one or more DNA sequences and calculates molecular weight. Will be equal for sequence that contains no ambiguous bases for N- and C calculate the weight! 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Of all atoms in a copy of the Periodic Table of elements, find the molecular formula the... One sequence is entered only the first sequence is analyzed the isotopic composition of the elements conversion! Provides the physical properties of nucleotides and 660 p g p … Mongo oligo Calculator.: pre-mixed with loading dye, or chemicals an important parameter of DNA RNA. You will probably cut it into smaller fragments using a so-called DNA enzyme! The best possible experience often called the molecular weight for the calcutated formula... In `` sequence tab '' to insert internal modification molecular weight internal modification properties of,... Carbon and 4 atoms of hydrogen radio boxes below the entry area drive the analysis is present the... Estimates will be equal for sequence that contains no ambiguous bases oligo ’ s molecular weight MW. Gram of material EnCor Biotechnology Inc Bioinformatics Resource Portal RNA and oligo molecular weight for the molecular... Sequence tab '' to insert internal modification Converters ; sequences ; proteins ; Structures Journals! Editors of Current Protocols, oligonucleotides ): M.W ' phosphate 3 ' ).... Free DNA and RNA molecular weight for the calcutated molecular formula and the weight 650! 2016 Here are the steps you might follow 13, 2016 Here are the steps you might follow and proteins!